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Proteins were detected using horseradish peroxidase (HRP)-conjugated IgG secondary antibody (#7074, Cell Signaling and 62-6520, Thermo Fisher Scientific) and SignalFire ECL Reagent (Cell Signaling)
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Dose or concentration:2.5 mg 0.5mg/ml 1ml
Introduction Type 2 diabetes mellitus (T2DM) represents a growing global health burden characterized by chronic hyperglycemia, insulin resistance, and progressive pancreatic β-cell dysfunction [1]
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