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Ileal protein lysates were extracted by homogenizing tissues in a lysis buffer (50 mM Tris [pH 8], 1 mM EDTA, 10% glycerol, 0.067% Brij 35) supplemented with protease inhibitors (MilliporeSigma) using a TissueLyzer II system (Qiagen)
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But studies Eng performed showed that the Gila monster’s hormone, exendin-4, doesn’t degrade for hours, making it a much better candidate for a drug
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