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Fourthly, Ghsr −/− and their wild-type mice fasted for 12 h (22:00–10:00) were administered ghrelin (1.5 nmol/50 µl saline, i.p.) at 10:00, then they were administered GLP-1 (3 nmol/50 µl saline, i.p.) or saline injection at 10:30, and food intake was measured from 10:30 to 11:30

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Single photon emission computed tomography (SPECT) images of the stomach were taken and processed to produce a 3- dimensional image allowing volume calculations