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A GPR6-rich brain region previously pharmacologically validated (striatum) was cut into 20 µm sections and placed in humified chambers for 120 min in 200 µl buffer (50 mM Tris buffer, pH 7.4 containing 50 mM NaCl, 6 mM MgCl 2, and 0.1% BSA) containing either 2.5 nM [ 3 H]-RL338 (total binding) and CVN527 at four different doses (1, 3, 10, 30 mg/kg) or 2.5 nM [ 3 H]-RL338 and 10 µM CVN527 (nonspecific binding)

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Protein deserves a place on your plate throughout the day